Coding

Part:BBa_K4632025:Design

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-10)


mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

What have we done? (SCAU-China 2023) We characterized its performance in E. coli TOP10. The characterization of reporter genes eGFP and RFP was performed simultaneously with the second verification system. Consistent results were obtained from SDS-PAGE experiments and q-RT-PCR experiments.

We hypothesize several possible reasons:

1. The selected reporter genes eGFP and RFP may not be suitable for expression in Top10.

2. Errors may have occurred in the design of the gene expression module.

3. Interactions between genes may have occurred.



Source

(Provided by Prof. Yongyao Xie, yyxie@scau.edu.cn).

References